KMID : 1004820100110020117
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Journal of Biomedical Research 2010 Volume.11 No. 2 p.117 ~ p.125
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Combination of TNF¥á?/ IFN¥ã Induces Apoptosis and IL-32 Expression in Human Colon Cancer Cell Line HCT116
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Cho Min-Chul
Nam Sang-Yoon Ahn Byeong-Woo Oh Ki-Wan Lee Beom-Jun Yun Young-Won Hong Jin-Tae
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Abstract
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Chronic inflammatory diseases such as Crohn¡Çs disease and ulcerative colitis are associated with increased risk of colon adenocarcinoma. Apoptic induction of colon cancer cells by cytokines and death receptors is an important anti-cancer therapy. We observed that co-administration of TNF¥á and IFN¥ã in human colon cancer cell line, HCT116, resulted in cell death and expression of IL-32. Cleavage forms of caspase-3, caspase-9, and PARP were increased in TNF¥á?/?IFN¥ã-treated HCT116. mRNA expression of death receptors, including TNFR1 and Fas were not changed and NO generation was not induced by combination of TNF¥á and IFN¥ã. However, mRNA expression of IL-32¥á, ¥â, and ¥ã was increased in TNF¥á?/?IFN¥ã-treated HCT116. To determine the effect of IL-32 in HCT116 cell apoptosis by TNF¥á?/?IFN¥ã stimulation, IL-32 siRNA-transfected HCT116 cells were cultured with TNF¥á?/?IFN¥ã and cell proliferation was measured. IL-32 siRNA induced slight recovery of cell viability of TNF¥á?/?IFN¥ã-stimulated HCT116. These results suggest that IL-32 is not directly related to apoptosis of HCT116 by TNF¥á?/?IFN¥ã stimulation. However, IL-32 expression by TNF¥á or TNF¥á?/?IFN¥ã in a colon cancer cell line is very interesting because of the unknown effect of IL-32 in colon cancer. Our study will contribute to development of studies for IL-32 function in human colon cancer and anti-cancer therapies using cytokines.
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KEYWORD
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HCT116 colon cancer cell line, TNF¥á, IFN¥ã, Apoptosis, IL-32
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